Resistance to Transformation by Insertionally Activated c-erbB Is a Dominant Phenotype in Fibroblasts

Tissue-specific factors influence whether cells are susceptible to transformation by erbB oncogenes. Avian fibroblasts resist transformation by insertionally activated c-erbB (IAc- erbB), whereas erythroblasts are transformed by this mutant of the epidermal growth factor receptor. In studies present...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1995-09, Vol.212 (1), p.277-283
Main Authors: Carter, T.H., Dominguez, N., Zeng, L., Kung, H.-J.
Format: Article
Language:English
Subjects:
3T3 Cells
Alleles
Animals
Base Sequence
Cell Transformation, Neoplastic - genetics
DNA Primers - chemistry
Fibroblasts
Genes, Dominant
Genes, erbB-2
Mice
Molecular Sequence Data
Oncogenes
Phenotype
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Summary:Tissue-specific factors influence whether cells are susceptible to transformation by erbB oncogenes. Avian fibroblasts resist transformation by insertionally activated c-erbB (IAc- erbB), whereas erythroblasts are transformed by this mutant of the epidermal growth factor receptor. In studies presented here, NIH/3T3 cells resisted transformation by IAc- erbB. This finding indicates that the nonpermissiveness of fibroblasts is conserved between avian and murine species. Surprisingly, expression of IAc- erbB blocked transformation by a v-erbB allele. The trans-dominant interference by IAc- erbB occurred despite expression at levels lower than for v- erbB. Together with previous reports, the results indicate that IAc- erbB can exert opposite growth effects in different tissues. The switch from positive to negative growth regulation, which depends on the cellular context, provides novel insight into tissue-specific regulation of receptor tyrosine kinases. Evasion of cell-specific inhibition apparently contributes to the distinct tissue tropisms of various erbB mutants.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1995.1484