Tau protein immunolocalization in fetal and adult human spinal cord

In the present study, the monoclonal antibody Alz-50 has been used to determine and compare the immunohistochemical localization of phosphorylated tau proteins in the developing and normal adult spinal cord. At all stages of fetal life Alz-50 fiber immunoreactivity was observed in the dorsal roots,...

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Bibliographic Details
Published in:Neuroscience research 1995-05, Vol.22 (2), p.197-202
Main Authors: Liberini, P., Valerio, A., Moretto, G., Rizzonelli, P., Memo, M., Rizzuto, N., Spano, P.F.
Format: Article
Language:English
Subjects:
Adult
Age Factors
Alz-50 monoclonal antibody
Antibodies, Monoclonal
Antibody Specificity
Development
Epitopes
Fetus
Fetus - chemistry
Human
Humans
Immunohistochemistry
Male
PHF-1 monoclonal antibody
Spinal cord
Spinal Cord - chemistry
Spinal Cord - embryology
Tau protein
tau Proteins - analysis
tau Proteins - immunology
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Summary:In the present study, the monoclonal antibody Alz-50 has been used to determine and compare the immunohistochemical localization of phosphorylated tau proteins in the developing and normal adult spinal cord. At all stages of fetal life Alz-50 fiber immunoreactivity was observed in the dorsal roots, in the dorsal and dorsolateral funiculi, and in restricted regions of the dorsal horn. Alz-50 immunoreactivity was also demonstrated in the dorsal root ganglion neurons. In the adult spinal cord a consistent pattern of Alz-50 fiber immunoreactivity was localized in the superficial layers of the dorsal horn (lamina I and II) but not in dorsal and dorsolateral funiculi and in the dorsal root ganglion. Comparable results in fetal specimens have been obtained employing PHF-1, a monoclonal antibody generated against paired helical filament proteins from Alzheimer brains, while no significant immunostaining for PHF-1 was observed in the adult spinal cord. In addition, the staining with monoclonal and polyclonal anti-tau antibodies overlapped with that of Alz-50. The transient, selective pattern of Alz-50 and PHF-1 immunoreactivity may disclose some relevant functions of tau proteins during somatosensory pathway development.
ISSN:0168-0102
1872-8111
DOI:10.1016/0168-0102(95)00897-2