Early Gene Signaling-Dependent and -Independent Induction of Apoptosis in Ramos Human B Cells Can Be Inhibited by over-Expression of BCL-2

We have previously shown that calcium ionophore-induced apoptosis of Ramos human B cells is preceded by the induced expression of early response genes, implying a requirement for new gene expression in this mode of programmed cell death. We have found in the present studies that inhibitors of macrom...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-10, Vol.215 (1), p.23-29
Main Authors: Ning, Z.Q., Norton, J.D., Johnson, D., Murphy, J.J.
Format: Article
Language:English
Subjects:
Apoptosis - drug effects
Apoptosis - genetics
B-Lymphocytes - physiology
Calcimycin - pharmacology
Cell Line
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Gene Expression
Humans
Kinetics
Protein Synthesis Inhibitors - pharmacology
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-bcl-2
Signal Transduction
Transfection
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Summary:We have previously shown that calcium ionophore-induced apoptosis of Ramos human B cells is preceded by the induced expression of early response genes, implying a requirement for new gene expression in this mode of programmed cell death. We have found in the present studies that inhibitors of macromolecular synthesis, cycloheximide and actinomycin D, are also potent inducers of apoptosis in the same Ramos cell model. These drugs trigger apoptosis through apparently early gene signalling-independent pathways. Although different mechanisms for induction of apoptosis exist in Ramos cells, enforced over-expression of Bcl-2 protects cells from apoptosis induced in response to different agents, demonstrating that Bcl-2 blocks a final common pathway for programmed cell death in the Ramos cell model.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2429