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Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication
Agricultural Biotechnology Center, Plant Science Institute, PO Box 411, 2101 Gödöllõ, Hungary The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis -acting sequences necessary for its replication by making a series of deletions throughou...
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| Published in: | Journal of general virology 1995-09, Vol.76 (9), p.2311-2316 |
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| Main Authors: | , , |
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| Language: | English |
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| container_end_page | 2316 |
| container_issue | 9 |
| container_start_page | 2311 |
| container_title | Journal of general virology |
| container_volume | 76 |
| creator | Havelda, Zoltan Dalmay, Tamas Burgyan, Jozsef |
| description | Agricultural Biotechnology Center, Plant Science Institute, PO Box 411, 2101 Gödöllõ, Hungary
The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis -acting sequences necessary for its replication by making a series of deletions throughout the 404 nt long molecule and testing the biological activity of mutants. Deletion or substitution of the conserved sequence blocks (A, B and C) always yielded inactive molecules. The deletion of only a few nucleotides could be tolerated beyond the natural deletion sites in blocks A and B. However, either half of block C1 (34 nt) and the first 25 nt of C2 (102 nt) could be deleted without loss of infectivity. It was also demonstrated that either one of the two halves of block C1 was specifically required for replication. We suggest that the last 77 nt of the viral genome and either half of block C1 represent the complementary strand promoter sequence recognized by the viral replicase.
* Author for correspondence. Fax +36 28 330482. e-mail burgyan@hubi.abc.hu
Received 28 February 1995;
accepted 9 May 1995. |
| doi_str_mv | 10.1099/0022-1317-76-9-2311 |
| format | article |
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The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis -acting sequences necessary for its replication by making a series of deletions throughout the 404 nt long molecule and testing the biological activity of mutants. Deletion or substitution of the conserved sequence blocks (A, B and C) always yielded inactive molecules. The deletion of only a few nucleotides could be tolerated beyond the natural deletion sites in blocks A and B. However, either half of block C1 (34 nt) and the first 25 nt of C2 (102 nt) could be deleted without loss of infectivity. It was also demonstrated that either one of the two halves of block C1 was specifically required for replication. We suggest that the last 77 nt of the viral genome and either half of block C1 represent the complementary strand promoter sequence recognized by the viral replicase.
* Author for correspondence. Fax +36 28 330482. e-mail burgyan@hubi.abc.hu
Received 28 February 1995;
accepted 9 May 1995.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-76-9-2311</identifier><identifier>PMID: 7561770</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Base Sequence ; Chromosome Mapping ; cymbidium ringspot virus ; Molecular Sequence Data ; RNA, Viral - biosynthesis ; RNA, Viral - genetics ; Sequence Deletion ; Tombusvirus - genetics ; Tombusvirus - physiology ; Virus Replication</subject><ispartof>Journal of general virology, 1995-09, Vol.76 (9), p.2311-2316</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-8cc54788b1c96d5ed700db62d584e1b4304dff1a2e582f5b9fb49fce1fd7f8da3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7561770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Havelda, Zoltan</creatorcontrib><creatorcontrib>Dalmay, Tamas</creatorcontrib><creatorcontrib>Burgyan, Jozsef</creatorcontrib><title>Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Agricultural Biotechnology Center, Plant Science Institute, PO Box 411, 2101 Gödöllõ, Hungary
The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis -acting sequences necessary for its replication by making a series of deletions throughout the 404 nt long molecule and testing the biological activity of mutants. Deletion or substitution of the conserved sequence blocks (A, B and C) always yielded inactive molecules. The deletion of only a few nucleotides could be tolerated beyond the natural deletion sites in blocks A and B. However, either half of block C1 (34 nt) and the first 25 nt of C2 (102 nt) could be deleted without loss of infectivity. It was also demonstrated that either one of the two halves of block C1 was specifically required for replication. We suggest that the last 77 nt of the viral genome and either half of block C1 represent the complementary strand promoter sequence recognized by the viral replicase.
* Author for correspondence. Fax +36 28 330482. e-mail burgyan@hubi.abc.hu
Received 28 February 1995;
accepted 9 May 1995.</description><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>cymbidium ringspot virus</subject><subject>Molecular Sequence Data</subject><subject>RNA, Viral - biosynthesis</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Deletion</subject><subject>Tombusvirus - genetics</subject><subject>Tombusvirus - physiology</subject><subject>Virus Replication</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS0EapeWT4Aq-YS4GOzEjp1jVbWAtAIJ0bPlP-OtURIvdrJV-fQ47KocOc3h_eaN5j2E3jL6gdG-_0hp0xDWMklkR3rStIy9QBvGO0Gaqr9Em2fiHL0u5SeljHMhz9CZFB2Tkm7Q4zY5M8TfZo5pwilgFwsxbo7TDhf4tcDkoGAoBaY5mgGHlLF7Gm30cRlxrljZpxnPabRLOcS8FOwhQDU4AI7TDDnASuHvX69xhv0Q3d9Tl-hVMEOBN6d5ge7vbn_cfCbbb5--3FxvieO0n4lyTnCplGWu77wALyn1tmu8UByY5S3lPgRmGhCqCcL2wfI-OGDBy6C8aS_Qu6PvPqf6TZn1GIuDYTATpKVoKQVva2T_BVmnlJLtCrZH0OVUSoag9zmOJj9pRvXai15T12vqWna612svdevqZL_YEfzzzqmIqr8_6g9x9_AYM-gdTGOsN2xMugb7z-oPaeeaWg</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Havelda, Zoltan</creator><creator>Dalmay, Tamas</creator><creator>Burgyan, Jozsef</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19950901</creationdate><title>Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication</title><author>Havelda, Zoltan ; Dalmay, Tamas ; Burgyan, Jozsef</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-8cc54788b1c96d5ed700db62d584e1b4304dff1a2e582f5b9fb49fce1fd7f8da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Base Sequence</topic><topic>Chromosome Mapping</topic><topic>cymbidium ringspot virus</topic><topic>Molecular Sequence Data</topic><topic>RNA, Viral - biosynthesis</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Deletion</topic><topic>Tombusvirus - genetics</topic><topic>Tombusvirus - physiology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Havelda, Zoltan</creatorcontrib><creatorcontrib>Dalmay, Tamas</creatorcontrib><creatorcontrib>Burgyan, Jozsef</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Havelda, Zoltan</au><au>Dalmay, Tamas</au><au>Burgyan, Jozsef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>76</volume><issue>9</issue><spage>2311</spage><epage>2316</epage><pages>2311-2316</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Agricultural Biotechnology Center, Plant Science Institute, PO Box 411, 2101 Gödöllõ, Hungary
The smallest defective interfering RNA (DI-2) of cymbidium ringspot tombusvirus (CyRSV) was used to identify the cis -acting sequences necessary for its replication by making a series of deletions throughout the 404 nt long molecule and testing the biological activity of mutants. Deletion or substitution of the conserved sequence blocks (A, B and C) always yielded inactive molecules. The deletion of only a few nucleotides could be tolerated beyond the natural deletion sites in blocks A and B. However, either half of block C1 (34 nt) and the first 25 nt of C2 (102 nt) could be deleted without loss of infectivity. It was also demonstrated that either one of the two halves of block C1 was specifically required for replication. We suggest that the last 77 nt of the viral genome and either half of block C1 represent the complementary strand promoter sequence recognized by the viral replicase.
* Author for correspondence. Fax +36 28 330482. e-mail burgyan@hubi.abc.hu
Received 28 February 1995;
accepted 9 May 1995.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>7561770</pmid><doi>10.1099/0022-1317-76-9-2311</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1995-09, Vol.76 (9), p.2311-2316 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77543146 |
| source | Freely Accessible Science Journals - check A-Z of ejournals |
| subjects | Base Sequence Chromosome Mapping cymbidium ringspot virus Molecular Sequence Data RNA, Viral - biosynthesis RNA, Viral - genetics Sequence Deletion Tombusvirus - genetics Tombusvirus - physiology Virus Replication |
| title | Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication |
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