Male and Female Cooperate in the Prohormone-like Processing of a Drosophila melanogaster Seminal Fluid Protein

Acp26Aa is a Drosophila seminal fluid protein that plays a role in the elevation of egg-laying by the mated female and has structural features of a prohormone. The protein, which has a region of sequence similarity to the egg-laying hormone of Aplysia, is transferred to the Drosophila female during...

Full description

Saved in:
Bibliographic Details
Published in:Developmental biology 1995-10, Vol.171 (2), p.694-702
Main Authors: Park, Morgan, Wolfner, Mariana F.
Format: Article
Language:English
Subjects:
ACCOUPLEMENT
Amino Acid Sequence
Animals
APARATO FEMENINO
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
COPULA
Diptera
DROSOPHILA MELANOGASTER
Drosophila melanogaster - physiology
Drosophilidae
Female
FEMELLE
Genitalia, Female - metabolism
GLANDE ANIMALE
GLANDULAS ANIMALES
HEMBRA
Invertebrate Hormones - metabolism
MACHO
MALE
Molecular Sequence Data
Molecular Weight
ORGANISME TRANSGENIQUE
Peptide Mapping
Protein Precursors - metabolism
PROTEINAS
PROTEINE
PROTEOLISIS
PROTEOLYSE
SECRECION
SECRETION
SEMEN
Semen - chemistry
Sexual Behavior, Animal
SPERME
SYSTEME GENITAL FEMELLE
TRANSGENICOS
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acp26Aa is a Drosophila seminal fluid protein that plays a role in the elevation of egg-laying by the mated female and has structural features of a prohormone. The protein, which has a region of sequence similarity to the egg-laying hormone of Aplysia, is transferred to the Drosophila female during mating. Acp26Aa is processed in the mated female's genital tract. We show here that the processing involves sequential proteolytic cleavages, and we map the position of these cleavages. Although Acp26Aa is not cleaved in the male, its processing in the mated female requires activities donated by the male. Acp26Aa ectopically expressed in unmated females is not processed. Processing of Acp26Aa in wild-type females mated to males with altered seminal fluid is dependent on the presence and amount of male accessory gland secretions. The need for molecular cooperation between the sexes for processing of Acp26Aa could restrict its activity to the mated female.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1995.1315