Rabbit anti-chromatin antibodies recognize similar epitopes on a histone H1 molecule as lupus autoantibodies

Autoantibodies in systemic lupus erythematosus (SLE) are believed to be produced through an antigen-driven mechanism, i.e., autosensitization with autoantigens. If this is the case, it is conceivable that antigenic determinants reactive with SLE autoantibodies and induced antibodies raised by immuni...

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Bibliographic Details
Published in:Clinical immunology and immunopathology 1995-10, Vol.77 (1), p.52-58
Main Authors: Morino, Noritsugu, Sakurai, Hiroyuki, Yamada, Akira, Yazaki, Yoshio, Minota, Seiji
Format: Article
Language:English
Subjects:
Animals
Autoantibodies - immunology
Autoantigens - immunology
Binding, Competitive
Biological and medical sciences
Chromatin - immunology
Epitope Mapping
Epitopes - immunology
Histones - immunology
Humans
Lupus Erythematosus, Systemic - immunology
Medical sciences
Rabbits
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
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Summary:Autoantibodies in systemic lupus erythematosus (SLE) are believed to be produced through an antigen-driven mechanism, i.e., autosensitization with autoantigens. If this is the case, it is conceivable that antigenic determinants reactive with SLE autoantibodies and induced antibodies raised by immunization are quite similar. To examine this issue, rabbits were immunized with purified histone H1 or chromatin. Although immunization with purified histone H1 did not produce detectable amounts of anti-histone H1 antibodies, immunization with chromatin mounted relatively good antibody responses against not only all histone subunits but also ssDNA. Epitopes on a histone H1 molecule reactive with SLE autoantibodies and rabbit antisera were determined using deletion mutants of a histone Hl molecule. At least two epitopes reactive with induced antibodies were found on a histone H1 and these were closely related to or the same as major autoepitopes in SLE. These data might indicate that B cells producing anti-histone Hl autoantibodies are quite similar to those induced by immunization and provide additional evidence that autosensitization with an autoantigen might be operative and a particulate antigen chromatin could be one of the immunogens for the production of anti-nuclear antibodies of various kinds in SLE.
ISSN:0090-1229
1090-2341
DOI:10.1016/0090-1229(95)90136-1