Rat intestinal crypt-cell replication factor with homology to early S-phase proteins required for cell division

Cell proliferation requires inhibitory and permissive factors to monitor cell-cycle progression and control DNA replication. The small intestine has a high rate of proliferation and a very low incidence of cancer, suggestive of efficient mechanisms for control of the cell cycle and assuring fidelity...

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Bibliographic Details
Published in:Gene 1995-10, Vol.163 (2), p.243-247
Main Authors: Sykes, Donald E., Weiser, Milton M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
cell cycle
Cell Cycle Proteins - biosynthesis
Cell Cycle Proteins - genetics
Cell Cycle Proteins - isolation & purification
Cell Division
Cell Line
Cloning, Molecular
Intestine, Small - cytology
Intestine, Small - metabolism
licensing factor
Molecular Sequence Data
Rats
Recombinant DNA
S Phase
Sequence Alignment
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Summary:Cell proliferation requires inhibitory and permissive factors to monitor cell-cycle progression and control DNA replication. The small intestine has a high rate of proliferation and a very low incidence of cancer, suggestive of efficient mechanisms for control of the cell cycle and assuring fidelity of DNA replication. We have isolated a cDNA from a rat crypt-cell library which hybridized to a 3.0-kb mRNA specific for crypt cells, the proliferative cell compartment of the intestine. Its amino-acid sequence indicates that it is a new member of a family of replication proteins found in yeast, Cenorhabditis elegans, mouse and humans. Its transcripts were markedly increased in fetal rat intestine and liver, decreased in long-term confluent and serum-starved tissue culture cells (IEC cells, a cell line derived from rat crypt cells), increased with serum repletion as cells resumed proliferation, and appeared to be species specific. Isolation and functional characterization of small intestinal crypt-cell replication factors should help explain this organ's low incidence of cancer.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00297-J