Effect of cyclosporin A on energy coupling in pea stem mitochondria

Effect of cyclosporin A on energy coupling in pea stem mitochondria is studied. It is found that incubation of mitochondria with 100 nM FCCP and/or CAtr, oligomycin, CaCl 2, palmitate and ADP results, after some lag phase, in a collapse of Δ/ψ generated by succinate oxidation in the presence of rote...

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Bibliographic Details
Published in:FEBS letters 1995-09, Vol.371 (3), p.258-260
Main Authors: Vianello, A., Macri, F., Braidot, E., Mokhova, E.N.
Format: Article
Language:English
Subjects:
Animals
Car☐yatractylate
Cyclosporin A
Cyclosporine - pharmacology
Dithioerythritol - pharmacology
Energy Metabolism - drug effects
Mitochondria - drug effects
Mitochondria - metabolism
Permeability transition
Pisum sativum - drug effects
Pisum sativum - metabolism
Plant mitochondria
Pora
Rats
Transmembrane electrical potentia
Uncouplers
Uncoupling Agents - pharmacology
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Summary:Effect of cyclosporin A on energy coupling in pea stem mitochondria is studied. It is found that incubation of mitochondria with 100 nM FCCP and/or CAtr, oligomycin, CaCl 2, palmitate and ADP results, after some lag phase, in a collapse of Δ/ψ generated by succinate oxidation in the presence of rotenone. Cyclosporin A (0.2 – 0.8 nmol/mg mitochondrial protein) markedly increases the lag phase. The cyclosporin A effect requires dithioerythritol to be added to the isolation medium. Metabisulphite fails to substitute for dithioerythritol. The relationships between these effects and cyclosporin A-sensitive mitochondrial permeability transition in animal mitochondria are discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00897-I