Genotype Study of Non-Hodgkin's Lymphoma

DNA from 47 patients with non-Hodgkin's lymphoma (NHL) was studied for immunoglobulin and T-cell receptor (TCR) gene rearrangements with Southern blot hybridization. In 83% of the cases the genotypic changes were consistent with immunophenotypic and morphologic examination. Two cases showed mix...

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Bibliographic Details
Published in:Pathology, research and practice research and practice, 1995-06, Vol.191 (5), p.444-455
Main Authors: Pitera, R., Pitera, J.E., Mufti, G.J., Salisbury, J.R.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
DNA Probes
DNA Restriction Enzymes
Female
Gene Rearrangement, B-Lymphocyte - genetics
Gene Rearrangement, T-Lymphocyte
Gene rearrangements
Genotype
Humans
Immunoglobulin genes
Lymphoma, B-Cell - genetics
Lymphoma, Non-Hodgkin - genetics
Lymphoma, T-Cell - genetics
Male
Middle Aged
Non-Hodgkin's lymphoma
Receptors, Antigen, T-Cell, alpha-beta - genetics
Receptors, Antigen, T-Cell, gamma-delta - analysis
Southern blot hybridization
T-cell receptor genes
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Summary:DNA from 47 patients with non-Hodgkin's lymphoma (NHL) was studied for immunoglobulin and T-cell receptor (TCR) gene rearrangements with Southern blot hybridization. In 83% of the cases the genotypic changes were consistent with immunophenotypic and morphologic examination. Two cases showed mixed genotype and 9 cases of B-cell NHL (67% of centroblastic, 36% of follicular and 33 % of large cell anaplastic) showed a population of cells with TCR gamma rearrangements in addition to immunoglobulin rearranged bands. We compared the TCR gamma variable region usage in these rearrangements in B-cell NHL with T -cell NHL and reactive hyperplasia. In T-cell NHL TCR gamma variable regions located at the 3' part of the variable locus were used more often, whilst in B-cell NHL regions of the 5' portion of the locus were preferentially used. Our results confirm the genotypic heterogeneity of histologically defined subtypes of NHL.
ISSN:0344-0338
1618-0631
DOI:10.1016/S0344-0338(11)80731-4