Involvement of Pim-1 in DNA Fragmentation in Mouse NS-1-Derived Cells

In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15 - 20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA (′ladder′)....

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-10, Vol.215 (2), p.538-546
Main Authors: Takahashi, C., Harada, Y., Ariga, H., Iguchiariga, S.M.M.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Base Sequence
Cell Line
Clone Cells
DNA Damage
DNA Primers
DNA Probes
DNA, Neoplasm - isolation & purification
DNA, Neoplasm - metabolism
Flow Cytometry
Gene Expression
Hybridomas
Mice
Molecular Sequence Data
Multiple Myeloma
Plasmids
Polymerase Chain Reaction
Protein-Serine-Threonine Kinases - metabolism
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-pim-1
Tumor Cells, Cultured
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Summary:In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15 - 20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA (′ladder′). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without ′ladder′. Transfection of a Pim-1 expression vector to a ′ladder′-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2498