RNA Expression and Chromosomal Location of the Mouse Long-Chain Acyl-CoA Dehydrogenase Gene

• Published in: Genomics (San Diego, Calif.) Calif.), 1995-07, Vol.28 (2), p.163-170
• Main Authors: Hinsdale, Myron E., Farmer, Susan C., Johnson, Kenneth R., Davisson, Muriel T., Hamm, Doug A., Tolwani, Ravi J., Wood, Philip A.
• Format: Article
• Language: English
• Subjects: Acyl-CoA Dehydrogenase, Long-Chain - biosynthesis; Acyl-CoA Dehydrogenase, Long-Chain - genetics; Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cloning, Molecular; Crosses, Genetic; DNA, Complementary - genetics; Enzyme Induction; Female; Fundamental and applied biological sciences. Psychology; Genes; Genes. Genome; Humans; Male; Mice - genetics; Mice, Inbred C57BL; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Muridae - genetics; Organ Specificity; Rats; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Transcription, Genetic
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.1127

The cDNA for mouse long-chain acyl-CoA dehydrogenase (Acadl, gene symbol; LCAD, enzyme) was cloned and characterized. The cDNA was obtained by library screening and reverse transcription-polymerase chain reaction (RT-PCR). The deduced amino acid sequence showed a high degree of homology to both the rat and the human LCAD sequence. Northern analysis of multiple tissues using the mouse Acadl cDNA as a probe showed two bands in all tissues examined. We found a total of three distinct mRNAs for Acadl. These three mRNAs were encoded by a single gene that we mapped to mouse chromosome 1. The three transcripts differed in the 3′ untranslated region due to use of alternative polyadenylation sites. Quantitative evaluation of a multitissue Northern blot showed a varied ratio of the larger transcript as compared with the smaller transcripts.