Identification of a novel human serpin gene; cloning sequencing and expression of leupin

A novel serpin gene has been isolated, cloned and sequenced. A PCR amplified fragment of the gene was originally identified from human genomic DNA, and the full-length cDNA was subsequently isolated from HeLa cells and sequenced. The novel serpin is very high in protein sequence similarity (91.8%) t...

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Bibliographic Details
Published in:FEBS letters 1995-10, Vol.373 (1), p.61-65
Main Authors: Barnes, Ruth C., Worrall, D.Margaret
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
cDNA sequence
Cloning, Molecular
DNA Primers
Gene Expression
HeLa cell
HeLa Cells
Hominidae - genetics
Humans
LEI, leukocyte elastase inhibitor
Leupin
Molecular Sequence Data
PAI-2, plasminogen activator inhibitor-2
Polymerase Chain Reaction
PTI, placental thrombin inhibitor
RT-PCR, reverse transcription-polymerase chain reaction
SCCA, squamous cell carcinoma antigen
Sequence Homology, Amino Acid
Serpin
Serpins - biosynthesis
Serpins - genetics
Squamous cell carcinoma antigen
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Summary:A novel serpin gene has been isolated, cloned and sequenced. A PCR amplified fragment of the gene was originally identified from human genomic DNA, and the full-length cDNA was subsequently isolated from HeLa cells and sequenced. The novel serpin is very high in protein sequence similarity (91.8%) to the squamous cell carcinoma antigen (SCCA), but contains substantial differences in the reactive site loop sequence, including a different amino acid (leucine) in the P 1 position. The gene product, named leupin, is expressed in HeLa cells, SKGIIIa cells and human placenta. The protein has a predicted M r of 44,857 and an isoelectric point of 6.04 which is consistent with the more acidic form of SCCA associated with squamous cell carcinomas.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01015-7