Four loci from human chromosome 1 are physically assigned to sheep chromosome 1p

• Published in: Mammalian genome 1995-08, Vol.6 (8), p.551-553
• Main Authors: Broad, T E, Burkin, D J, Cambridge, L M, Carpenter, M A, Jones, C
• Format: Article
• Language: English
• Subjects: alpha-Amylases - genetics; Animals; CD3 Complex - genetics; Chromosome Mapping; Chromosomes, Human, Pair 1; Cricetinae; Humans; Hybrid Cells; Nerve Growth Factors - genetics; Phosphoglucomutase - genetics; Sheep - genetics; Sodium-Potassium-Exchanging ATPase - genetics; Thyrotropin - genetics; Urate Oxidase - genetics
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/BF00356176

Phosphoglucomutase 1 (PGM1), thyroid stimulating hormone beta (TSHB), and urate oxidase (UOX) were newly assigned to sheep Chromosome (Chr) 1p by Southern hybridization to DNA from a minipanel of sheep x hamster cell hybrids. The location of antigen CD3, zeta polypeptide (CD3Z) on sheep Chr 1p was confirmed with the same cell hybrids. These assignments support the suggestion that sheep Chr 1p and its cattle homolog Chr 3 were derived from a chromosomal segment with linkage homology to a large region of human Chr 1. Sheep Chr 1p comprises about 5% of the sheep genome but has few loci physically assigned to it, reflecting the influence of the cattle gene map on the mapping of the sheep genome. Thus, the cattle map, as of April 1993, showed no loci assigned to cattle Chr 3, which is the banded homolog of sheep Chr 1p (ISCNDA 1989). Only one of the 107 loci on the sheep genome had been mapped on sheep Chr 1p by the end of June 1993. The location of a nucleolus organizer region (RNR1) on sheep Chr 1pter, established cytologically by several researchers since 1977, was confirmed by Phua and associates by in situ hybridization. Using this procedure, we have recently assigned nerve growth factor beta (NGFB) and CD3Z to sheep Chr 1p24-p21 and 1p14-p11 respectively. NGFB was reported by Fries and colleagues to be a member of the cattle syntenic group U6, but CD3Z has not yet been mapped in cattle. Thus, our mapping of NGFB in sheep suggested that the cattle U6 syntenic group could be assigned to cattle Chr 3. Fries and coworkers listed four other loci in cattle U6 as well as NGFB, all of which can now be regarded as putative sheep 1p loci. These are chymosin (CYM), heat shock 70-kDa protein 4 (HSP70-4), phosphoglucomutase 1 (PGM1), and DNA segment: (ETH1112) (DU6S1). Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), amylase alpha 1A; salivary (AMY1) and amylase alpha 2A; pancreatic (AMY2) have also been reported by O'Brien and associates to be in cattle syntenic group U6. More recently, erythrocyte antigen L (EAL); Fc fragment of IgG, low affinity II, receptor (FCGR2); and DNA segment: (INRA197) (D3S26) have also been physically assigned to cattle Chr 3 (U6). The loci PGM1, AMY1/2, NGFB, and NRAS are all found in the proximal region of human Chr 1p. CD3Z, now localized on sheep 1p, is known to be on human Chr 1q22-q25. These data suggested that sheep Chr 1p and its cattle homolog Chr 3 may be made up of a large conserved segment (spanning the centromere) of h