Parathyroid hormone-related peptide and 8701-BC breast cancer cell growth and invasion in vitro: evidence for growth-inhibiting and invasion-promoting effects

It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 87...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 1995-06, Vol.111 (2), p.225-232
Main Authors: Luparello, Claudio, Burtis, William J, Raue, Friedhelm, Birch, Mark A., Gallagher, James A.
Format: Article
Language:English
Subjects:
8701-BC cells
Base Sequence
Breast cancer
Breast Neoplasms - pathology
Cell Division - drug effects
Cell invasion
Cell proliferation
Endopeptidases - metabolism
Humans
Molecular Sequence Data
Neoplasm Invasiveness
Parathyroid Hormone - genetics
Parathyroid hormone-related peptide
Parathyroid Hormone-Related Protein
Polymerase Chain Reaction
Protease Inhibitors - pharmacology
Proteins - pharmacology
Receptor, Parathyroid Hormone, Type 1
Receptors, Parathyroid Hormone - genetics
RNA, Messenger - metabolism
RNA-Directed DNA Polymerase
Tumor Cells, Cultured
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Summary:It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 8701-BC cell medium using antibodies raised against midregion and C-terminal fragments, and also demonstrated the expression of PTH PTHrP receptor by 8701-BC cells. Secondly, we have examined the role, if any, elicited by diverse PTHrP domains on 8701-BC cell proliferation, and invasive behaviour in vitro related to production of extracellular proteolytic enzymes. Our data show that PTHrP [1–34], and, to a minor extent, [67–86] and [107–139], are anti-mitogenic but ‘invadogenic’ for 8701-BC cells, and suggest that diverse enzymatic activities may contribute to cell invasion in response to different PTHrP fragments. In light of the present data on a chemoattractive role for PTHrP in vitro, we hypothesize that this protein might intervene in local control of the invasive process in breast carcinoma.
ISSN:0303-7207
1872-8057
DOI:10.1016/0303-7207(95)03577-T