Nucleotide-sequence analysis indicates that a DNA plasmid in a diseased isolate of Ophiostoma novo-ulmi is derived by recombination between two long repeat sequences in the mitochondrial large subunit ribosomal RNA gene

The nucleotide sequence of a mitochondrial plasmid (2234 bp) in a diseased isolate of Ophiostoma novo-ulmi, and sequences of the mitochondrial DNA that overlap and flank the plasmid end-points, have been determined. The plasmid was shown to be derived from the 0. novo-ulmi mitochondrial large subuni...

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Bibliographic Details
Published in:Current genetics 1995-06, Vol.28 (1), p.54-59
Main Authors: Abu-Amero, S.N, Charter, N.W, Buck, K.W, Brasier, C.M. (Imperial Coll. of Science, Technology and Medicine, London (United Kingdom). Dept. of Biology)
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Ascomycota - genetics
Base Sequence
DNA plasmid
DNA, Fungal
DNA, Mitochondrial
Exons
GENE
GENES
Introns
Mitochondrial large subunit ribosomal RNA gene
Molecular Sequence Data
Open Reading Frames
OPHIOSTOMA
Ophiostoma novo-ulmi
Pflanzenzuechtung
PLASMIDE
PLASMIDIOS
Plasmids
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
Repetitive Sequences, Nucleic Acid
RNA, Ribosomal - genetics
SECUENCIA NUCLEOTIDICA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEOTIDIQUE
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Summary:The nucleotide sequence of a mitochondrial plasmid (2234 bp) in a diseased isolate of Ophiostoma novo-ulmi, and sequences of the mitochondrial DNA that overlap and flank the plasmid end-points, have been determined. The plasmid was shown to be derived from the 0. novo-ulmi mitochondrial large subunit ribosomal RNA gene and contained most of intron 1, the whole of exon 2, and probably the first part of intron 2. Within intron 1 there is an open reading frame with the potential to encode a 323 amino-acid polypeptide which contained dodecapeptide sequences typical of RNA maturases and DNA endonucleases. The endpoints of the plasmid in the mtDNA were located within two 90-bp direct imperfect repeat sequences, one of which comprised the last 7 bp of exon 1 and the first 83 bp of intron 1 whilst the other comprised the last 7 bp of exon 2 and the first 83 bp of intron 2. It is proposed that the Ld plasmid was generated by intramolecular recombination between these two repeats with the crossover point probably within the last 15 bp.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00311881