Antibodies against murine double minute-2 (mdm2) in sera of patients with various gynaecological diseases

We performed a serologic analysis for anti-murine double minute-2 (anti-mdm2) antibodies in sera of patients with different gynaecological diseases using immunoblotting technique with recombinant mdm2 as antigen. In addition, for large scale screening we established an anti-mdm2 enzyme-linked immuno...

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Bibliographic Details
Published in:Cancer letters 1995-09, Vol.96 (1), p.111-115
Main Authors: Selter, Helga, Amela-Neuschwander, Sara, Villena-Heinsen, Carlos, Montenarh, Mathias
Format: Article
Language:English
Subjects:
Autoantibodies - immunology
Biological and medical sciences
Breast Neoplasms - immunology
Enzyme-Linked Immunosorbent Assay
Female
Female genital diseases
Genital Diseases, Female - immunology
Gynaecological diseases
Gynecology. Andrology. Obstetrics
Humans
mdm-2 antibodies
Medical sciences
Neoplasm Proteins - immunology
Nuclear Proteins
Proto-Oncogene Proteins - immunology
Proto-Oncogene Proteins c-mdm2
Recombinant Proteins - immunology
Serologic analysis
Tumor Suppressor Protein p53 - immunology
Tumors
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Summary:We performed a serologic analysis for anti-murine double minute-2 (anti-mdm2) antibodies in sera of patients with different gynaecological diseases using immunoblotting technique with recombinant mdm2 as antigen. In addition, for large scale screening we established an anti-mdm2 enzyme-linked immunosorbent assay (ELISA). Serum samples from patients with breast cancer, ovarian carcinoma, cervix carcinoma and benign gynaecological tissue alterations were tested. We detected antibodies specific for mdm2 in sera derived from cancer patients, as well as from patients with non-malignant diseases. Some of the sera with antibodies against mdm2 also contained antibodies against the growth suppressor gene product p53.
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(95)03920-R