Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35

The baculovirus antiapoptotic protein p35 inhibited the proteolytic activity of human interleukin-1 beta converting enzyme (ICE) and three of its homologs in enzymatic assays. Coexpression of p35 prevented the autoproteolytic activation of ICE from its precursor form and blocked ICE-induced apoptosi...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1995-09, Vol.269 (5232), p.1885-1888
Main Authors: Bump, N.J. (BASF Bioresearch Corporation, Worchester, MA.), Hackett, M, Hugunin, M, Seshagiri, S, Brady, K, Chen, P, Ferenz, C, Franklin, S, Ghayur, T, Li, P
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Ageing, cell death
Amino Acid Sequence
Animals
Apoptosis
BACULOVIRIDAE
baculovirus
Binding Sites
Binding, Competitive
Biological and medical sciences
Caspase 1
Cell death
Cell Line
Cell physiology
CELLULE
CELULAS
CRECIMIENTO
CROISSANCE
Cysteine Endopeptidases - metabolism
Cysteine Proteinase Inhibitors - genetics
Cysteine Proteinase Inhibitors - metabolism
Cysteine Proteinase Inhibitors - pharmacology
Enzyme Activation - drug effects
Enzymes
Fundamental and applied biological sciences. Psychology
Humans
INHIBICION
INHIBIDORES DE ENZIMAS
INHIBITEUR D'ENZYME
INHIBITION
Inhibitor of Apoptosis Proteins
Logical Thinking
Molecular and cellular biology
Molecular Sequence Data
MORT
MUERTE
PROTEASAS
PROTEASE
Proteases
PROTEINAS
PROTEINE
Proteins
Recombinant Proteins - pharmacology
Stoichiometry
Transfection
Viral Proteins - genetics
Viral Proteins - metabolism
Viral Proteins - pharmacology
Viruses
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Description
Summary:The baculovirus antiapoptotic protein p35 inhibited the proteolytic activity of human interleukin-1 beta converting enzyme (ICE) and three of its homologs in enzymatic assays. Coexpression of p35 prevented the autoproteolytic activation of ICE from its precursor form and blocked ICE-induced apoptosis. Inhibition of enzymatic activity correlated with the cleavage of p35 and the formation of a stable ICE-p35 complex. The ability of p35 to block apoptosis in different pathways and in distantly related organisms suggests a central and conserved role for ICE-like proteases in the induction of apoptosis
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7569933