Identification of Leishmania chagasi Antigens Recognized by Human Lymphocytes

Preparative SDS-PAGE followed by electroelution was used to separate proteins of Leishmania chagasi promastigotes into 67 fractions. These fractions were tested for the ability to stimulate proliferation of peripheral blood mononuclear cells from healthy immune donors who were treated previously for...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-10, Vol.172 (4), p.1055-1060
Main Authors: Jeronimo, Selma M. B., Higgs, Elizabeth, Vedvick, Thomas, Mann, Barbara J., Jernigan, John, Petri, William A., Pearson, Richard D.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens
Antigens, Protozoan - immunology
Antigens, Protozoan - isolation & purification
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gels
Heat shock proteins
Humans
Infections
Leishmania chagasi
Leishmaniasis
Leishmaniasis, Visceral - immunology
Lymphocyte Activation
Lymphocytes
Major Articles
Molecular Sequence Data
Parasites
Promastigotes
Protozoa
Protozoan Proteins - immunology
Protozoan Proteins - isolation & purification
Sequence Homology, Amino Acid
T lymphocytes
Visceral leishmaniasis
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Summary:Preparative SDS-PAGE followed by electroelution was used to separate proteins of Leishmania chagasi promastigotes into 67 fractions. These fractions were tested for the ability to stimulate proliferation of peripheral blood mononuclear cells from healthy immune donors who were treated previously for visceral leishmaniasis and from nonimmune controls. The proliferative responses elicited by these proteins varied among individuals. The 69-kDa protein fraction contained a fragment with sequence similarity to the 70-kDa heat-shock protein. Fragments of the 46- and 41-kDa fractions had sequences not present in the National Biological Research Foundation data bank. These data suggest that a successful subunit vaccine may require multiple parasite antigens. The identification of antigens that elicit human T cell responses is an important step toward understanding the immunology of L. chagasi infection and ultimately in the development of a vaccine.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/172.4.1055