The drosophila melanogaster homologue of the human BBC1 gene is highly expressed during embryogenesis

• Published in: Gene 1995-09, Vol.162 (2), p.245-248
• Main Authors: Helps, N.R., Adams, S.M., Brammar, W.J., Varley, J.M.
• Format: Article
• Language: English
• Subjects: 0.15 M NaCl/0.015 M Na3·citrate pH 7.6; Amino Acid Sequence; amino acid sequences; amino acid(s); Animals; Arabidopsis; Arabidopsis thaliana; base pair(s); Base Sequence; BBC1; bbcl (human BBC1); bbcl product; Brassica; Brassica napus; breast basic conserved 1 gene; Breast tumor; cDNA; Cloning, Molecular; complementary DNA; D. melanogaster; deoxyribonucleoside triphosphate; development; DNA complementary to RNA; DNA, Complementary - genetics; dNTP; Drosophila melanogaster; Drosophila melanogaster - embryology; Drosophila melanogaster - genetics; Drosophila Proteins; embryogenesis; Female; genbank/x77926; gene expression; Gene Expression Regulation, Developmental; Genes, Insect; hour; Introns; isoelectric point; kilobase(s) or 1000 bp; Male; messenger RNA; Molecular Sequence Data; Neoplasm Proteins - genetics; NLS; nuclear localisation sequence(s); nucleotide sequences; nucleotide(s); open reading frame; open reading frames; ORF; Oryza sativa L; PCR; polymerase chain reaction; proteins; RACE; rapid amplification of cDNA ends; rat; ribosomal; ribosomal protein; ribosomal protein l13; Ribosomal Proteins - genetics; ribosomes; RNA, Messenger - genetics; Sequence Alignment; Sequence Homology, Amino Acid; SSC; untranslated region(s); UTR
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(95)00356-B

We have previously reported the isolation and preliminary characterisation of a full-length cDNA sequence derived from the human BBC1 gene, a gene which displays differential expression in tumours of the female breast [Adams et al., Hum. Mol. Genet. 1 (1992) 91-96]. Here, we report the isolation and characterisation of the Drosophila melanogaster (Dm) homologue of this human gene. The Dmbbcl cDNA is 62% identical to the human BBC1 cDNA within a conserved open reading frame and the encoded proteins share 74% sequence similarity. The Dmbbcl mRNA is expressed at all stages of Dm development, with the highest levels of expression occurring during embryogenesis. In addition, the Dm and human BBC1 proteins share remarkable degrees of identity with the products of recently isolated plant and avian bbcl cDNAs. The sequences of all the predicted BBC1 proteins are highly similar to that of the rat ribosomal subunit protein L13 [Olvera and Wool, Biochem. Biophys. Res. Commun. 201 (1994) 102-107], strongly indicating that the BBC1 protein is ribosomal protein L13.