Hepatic apolipoprotein and LDL receptor gene expression in the genetically hypercholesterolemic (RICO) rat

The present study was designed to examine apolipoprotein and LDL receptor gene expression in genetically hypercholesterolemic RICO rats. In the plasma of RICO rats as compared to SW (control) rats, the hypercholesterolemia (+ 41%) was associated with a significant increase in plasma apo B (+ 23%) an...

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Bibliographic Details
Published in:Atherosclerosis 1995-09, Vol.117 (1), p.15-24
Main Authors: Felgines, Catherine, Sérougne, Colette, Mazur, Andrzej, Férézou, Jacqueline, Lutton, Claude, Rayssiguier, Yves
Format: Article
Language:English
Subjects:
Adrenal Glands - metabolism
Adrenals
Animals
Apolipoprotein
Apolipoproteins - genetics
Apolipoproteins - metabolism
Biological and medical sciences
Cholesterol
Disease Models, Animal
Disorders of blood lipids. Hyperlipoproteinemia
Gene Expression
Hypercholesterolemia - genetics
Hypercholesterolemia - metabolism
LDL receptor
Liver
Liver - metabolism
Male
Medical sciences
Metabolic diseases
Rats
Receptors, LDL - genetics
Receptors, LDL - metabolism
RICO rat
RNA, Messenger - analysis
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Summary:The present study was designed to examine apolipoprotein and LDL receptor gene expression in genetically hypercholesterolemic RICO rats. In the plasma of RICO rats as compared to SW (control) rats, the hypercholesterolemia (+ 41%) was associated with a significant increase in plasma apo B (+ 23%) and apo E (+ 68%) concentrations. Study of apolipoprotein synthesis in the liver has shown that this increase in plasma apo B and apo E concentrations was not associated with modification in their synthesis and mRNA levels. Study of apo E mRNA level in various tissues has shown only the modification in adrenals in RICO as compared to SW rats (2.7-fold increase). Study of LDL binding, LDL receptor mass and LDL receptor mRNA level in the liver of RICO and SW rats has shown no significant differences between these two strains. EDTA-resistant binding of rat LDL was lower in RICO than in SW rats suggesting that binding sites others than the LDL receptor are present in lesser amount in this hypercholesterolemic strain.
ISSN:0021-9150
1879-1484
DOI:10.1016/0021-9150(95)05545-8