A high efficiency method for purification and assay of bee venom phospholipase A2

Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving...

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Bibliographic Details
Published in:Pathology 1995, Vol.27 (2), p.157-160
Main Authors: Ameratunga, R.V., Hawkins, R., Prestidge, R., Marbrook, J.
Format: Article
Language:English
Subjects:
alkaline picrate
Allergological tests
Animals
Bee Venoms - chemistry
Biological and medical sciences
Biological Assay - methods
bipyridylium
Blotting, Western
Chromatography, High Pressure Liquid
diquat
Electrophoresis, Polyacrylamide Gel
Guinea Pigs
Hemolysis
Immunological methods for diagnosis and exploration
Immunopathology
In Vitro Techniques
interference
Medical sciences
Paraquat
Phospholipases A - analysis
Phospholipases A - isolation & purification
Phospholipases A2
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Summary:Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving the hemolytic action of PLA2. In addition we describe a simple functional assay of PLA2 based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA2 of venoms which are available in limited quantities.
ISSN:0031-3025
1465-3931
DOI:10.1080/00313029500169782