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A high efficiency method for purification and assay of bee venom phospholipase A2
Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving...
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| Published in: | Pathology 1995, Vol.27 (2), p.157-160 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c429t-ba28df752d625a647040f60f7fe48a51afbbfb9043cff22e6c616f22388d58ca3 |
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| cites | cdi_FETCH-LOGICAL-c429t-ba28df752d625a647040f60f7fe48a51afbbfb9043cff22e6c616f22388d58ca3 |
| container_end_page | 160 |
| container_issue | 2 |
| container_start_page | 157 |
| container_title | Pathology |
| container_volume | 27 |
| creator | Ameratunga, R.V. Hawkins, R. Prestidge, R. Marbrook, J. |
| description | Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving the hemolytic action of PLA2. In addition we describe a simple functional assay of PLA2 based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA2 of venoms which are available in limited quantities. |
| doi_str_mv | 10.1080/00313029500169782 |
| format | article |
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This method removes all detectable contaminants such as melittin from PLA2 while preserving the hemolytic action of PLA2. In addition we describe a simple functional assay of PLA2 based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA2 of venoms which are available in limited quantities.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.1080/00313029500169782</identifier><identifier>PMID: 7567144</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>alkaline picrate ; Allergological tests ; Animals ; Bee Venoms - chemistry ; Biological and medical sciences ; Biological Assay - methods ; bipyridylium ; Blotting, Western ; Chromatography, High Pressure Liquid ; diquat ; Electrophoresis, Polyacrylamide Gel ; Guinea Pigs ; Hemolysis ; Immunological methods for diagnosis and exploration ; Immunopathology ; In Vitro Techniques ; interference ; Medical sciences ; Paraquat ; Phospholipases A - analysis ; Phospholipases A - isolation & purification ; Phospholipases A2</subject><ispartof>Pathology, 1995, Vol.27 (2), p.157-160</ispartof><rights>1995 Royal College of Pathologists of Australasia</rights><rights>1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-ba28df752d625a647040f60f7fe48a51afbbfb9043cff22e6c616f22388d58ca3</citedby><cites>FETCH-LOGICAL-c429t-ba28df752d625a647040f60f7fe48a51afbbfb9043cff22e6c616f22388d58ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3633926$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7567144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ameratunga, R.V.</creatorcontrib><creatorcontrib>Hawkins, R.</creatorcontrib><creatorcontrib>Prestidge, R.</creatorcontrib><creatorcontrib>Marbrook, J.</creatorcontrib><title>A high efficiency method for purification and assay of bee venom phospholipase A2</title><title>Pathology</title><addtitle>Pathology</addtitle><description>Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving the hemolytic action of PLA2. In addition we describe a simple functional assay of PLA2 based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA2 of venoms which are available in limited quantities.</description><subject>alkaline picrate</subject><subject>Allergological tests</subject><subject>Animals</subject><subject>Bee Venoms - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - methods</subject><subject>bipyridylium</subject><subject>Blotting, Western</subject><subject>Chromatography, High Pressure Liquid</subject><subject>diquat</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Guinea Pigs</subject><subject>Hemolysis</subject><subject>Immunological methods for diagnosis and exploration</subject><subject>Immunopathology</subject><subject>In Vitro Techniques</subject><subject>interference</subject><subject>Medical sciences</subject><subject>Paraquat</subject><subject>Phospholipases A - analysis</subject><subject>Phospholipases A - isolation & purification</subject><subject>Phospholipases A2</subject><issn>0031-3025</issn><issn>1465-3931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kU2L1TAUhoMo453RH-BCyELcVfPdFFeXQR1hQARdl9P0xGZom5q0A_ffm_GW2QizCAl5n_cQnhDyhrMPnFn2kTHJJRONZoybprbiGTlwZXQlG8mfk8NDXhVAvySXOd8xxpS19oJc1NrUXKkD-XGkQ_g9UPQ-uICzO9EJ1yH21MdEly2Fcg9riDOFuaeQM5xo9LRDpPc4x4kuQ8xljWGBjPQoXpEXHsaMr_f9ivz68vnn9U11-_3rt-vjbeWUaNaqA2F7X2vRG6HBqJop5g3ztUdlQXPwXee7hinpvBcCjTPclIO0ttfWgbwi789zlxT_bJjXdgrZ4TjCjHHLbV3rRtTSFpCfQZdizgl9u6QwQTq1nLUPGtv_NJbO23341k3YPzZ2byV_t-eQHYw-wexCfsSkkbIRpmCfzliYi84JBoRxHRwkbO_ilubi58lH7G0sGu8Dpjb_-yLsQ0K3tn0MT7T_Au7-nhk</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Ameratunga, R.V.</creator><creator>Hawkins, R.</creator><creator>Prestidge, R.</creator><creator>Marbrook, J.</creator><general>Elsevier B.V</general><general>Informa UK Ltd</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>A high efficiency method for purification and assay of bee venom phospholipase A2</title><author>Ameratunga, R.V. ; Hawkins, R. ; Prestidge, R. ; Marbrook, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-ba28df752d625a647040f60f7fe48a51afbbfb9043cff22e6c616f22388d58ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>alkaline picrate</topic><topic>Allergological tests</topic><topic>Animals</topic><topic>Bee Venoms - chemistry</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - methods</topic><topic>bipyridylium</topic><topic>Blotting, Western</topic><topic>Chromatography, High Pressure Liquid</topic><topic>diquat</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Guinea Pigs</topic><topic>Hemolysis</topic><topic>Immunological methods for diagnosis and exploration</topic><topic>Immunopathology</topic><topic>In Vitro Techniques</topic><topic>interference</topic><topic>Medical sciences</topic><topic>Paraquat</topic><topic>Phospholipases A - analysis</topic><topic>Phospholipases A - isolation & purification</topic><topic>Phospholipases A2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ameratunga, R.V.</creatorcontrib><creatorcontrib>Hawkins, R.</creatorcontrib><creatorcontrib>Prestidge, R.</creatorcontrib><creatorcontrib>Marbrook, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ameratunga, R.V.</au><au>Hawkins, R.</au><au>Prestidge, R.</au><au>Marbrook, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A high efficiency method for purification and assay of bee venom phospholipase A2</atitle><jtitle>Pathology</jtitle><addtitle>Pathology</addtitle><date>1995</date><risdate>1995</risdate><volume>27</volume><issue>2</issue><spage>157</spage><epage>160</epage><pages>157-160</pages><issn>0031-3025</issn><eissn>1465-3931</eissn><abstract>Phosphotipase A2 (PLA2) is the main allergen of hymenopteran venoms, We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA2 from crude bee venom. This method removes all detectable contaminants such as melittin from PLA2 while preserving the hemolytic action of PLA2. In addition we describe a simple functional assay of PLA2 based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA2 of venoms which are available in limited quantities.</abstract><cop>London</cop><pub>Elsevier B.V</pub><pmid>7567144</pmid><doi>10.1080/00313029500169782</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-3025 |
| ispartof | Pathology, 1995, Vol.27 (2), p.157-160 |
| issn | 0031-3025 1465-3931 |
| language | eng |
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| source | Elsevier |
| subjects | alkaline picrate Allergological tests Animals Bee Venoms - chemistry Biological and medical sciences Biological Assay - methods bipyridylium Blotting, Western Chromatography, High Pressure Liquid diquat Electrophoresis, Polyacrylamide Gel Guinea Pigs Hemolysis Immunological methods for diagnosis and exploration Immunopathology In Vitro Techniques interference Medical sciences Paraquat Phospholipases A - analysis Phospholipases A - isolation & purification Phospholipases A2 |
| title | A high efficiency method for purification and assay of bee venom phospholipase A2 |
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