Radiosensitivity of mouse myeloid stem cells in culture after stimulation by macrophage colony-stimulating factor

Mouse bone marrow cells were plated in a semi-solid medium containing an excess amount of macrophage colony-stimulating factor (M-CSF). The stimulated macrophage colony-forming cells (CFU-M) took about 40 hours before dividing into two cells. X-Ray irradiation of the cells shortly after plating decr...

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Bibliographic Details
Published in:Journal of radiation research 1987, Vol.28 (1), p.126-134
Main Authors: Sugavara, S, Tsuneoka, K, Ohara, H, Shikita, M
Format: Article
Language:English
Subjects:
Animals
Hematopoietic Stem Cells - drug effects
Hematopoietic Stem Cells - radiation effects
In Vitro Techniques
Interleukin-3 - pharmacology
Mice
Radiation Tolerance
Stimulation, Chemical
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Summary:Mouse bone marrow cells were plated in a semi-solid medium containing an excess amount of macrophage colony-stimulating factor (M-CSF). The stimulated macrophage colony-forming cells (CFU-M) took about 40 hours before dividing into two cells. X-Ray irradiation of the cells shortly after plating decreased the survival of CFU-M, while the quasithreshold dose (Dq) and the 37% dose slope (Do) of the dose-response curve were 0.54 Gy and 1.27 Gy. Irradiation 5-50 hours after plating decreased CFU-M survival to the same extent as irradiation shortly after plating. Furthermore, the survival rate of CFU-M was similar to the above when the cells were irradiated in a liquid medium containing no M-CSF. These results suggest that the radiosensitivity of CFU-M does not notably change after their stimulation by M-CSF.
ISSN:0449-3060
1349-9157
DOI:10.1269/jrr.28.126