A novel quantitative receptor autoradiography and in situ hybridization histochemistry technique using storage phosphor screen imaging

A new technique of image acquisition for quantitative receptor autoradiography and in situ hybridization histochemistry was developed using storage phosphor screen imaging. This method was at least 4–5 times faster than conventional film densitometry. Two of the advantages of the phosphor screen met...

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Bibliographic Details
Published in:Journal of neuroscience methods 1995-07, Vol.59 (2), p.265-271
Main Authors: Ito, T., Suzuki, T., Lim, D.K., Wellman, S.E., Ho, I.K.
Format: Article
Language:English
Subjects:
[ 35S]t-Butylbicyclophosphorothionate
Animals
Autoradiography - methods
Biological and medical sciences
Brain - anatomy & histology
Brain - metabolism
Bridged Bicyclo Compounds, Heterocyclic
Calibration
Densitometry
Deoxyadenine Nucleotides - metabolism
Digital imaging
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Histocytochemistry - methods
Image Processing, Computer-Assisted - methods
In Situ Hybridization - methods
In situ hybridization histochemistry
Male
Oligonucleotide Probes
Rats
Rats, Sprague-Dawley
Receptor autoradiography
Receptors, GABA-A - drug effects
Receptors, GABA-A - metabolism
RNA, Messenger - metabolism
Storage phosphor screen
Vertebrates: nervous system and sense organs
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Summary:A new technique of image acquisition for quantitative receptor autoradiography and in situ hybridization histochemistry was developed using storage phosphor screen imaging. This method was at least 4–5 times faster than conventional film densitometry. Two of the advantages of the phosphor screen method are high sensitivity and wide linear range of response. Other aspects of this method were compared with those of conventional densitometry. Use of storage phosphor screen imaging will allow greatly increased speed of pharmacological screening procedures that utilize quantitative autoradiography.
ISSN:0165-0270
1872-678X
DOI:10.1016/0165-0270(94)00212-Y