Synergistic Neutralization of Rubella Virus by Monoclonal Antibodies to Viral Haemagglutinin

1 Virus Laboratory, Institute of Infectious Diseases, University of Pavia, IRCCS Policlinico S. Matteo, 27100 Pavia and 2 Central Research Laboratory, Sorin Biomedica SpA, 13040 Saluggia, Italy Using murine monoclonal antibodies (MAbs) to rubella virus haemagglutinin, five epitopes were identified i...

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Bibliographic Details
Published in:Journal of general virology 1987-07, Vol.68 (7), p.2007-2012
Main Authors: Gerna, Giuseppe, Revello, M. Grazia, Dovis, Mauro, Petruzzelli, Eleonora, Achilli, Giorgio, Percivalle, Elena, Torsellini, Maria
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigens, Viral - immunology
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Glycoproteins - immunology
Hemagglutinins, Viral - immunology
Mice
Mice, Inbred BALB C - immunology
Microbiology
Neutralization Tests
rubella virus
Rubella virus - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
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Summary:1 Virus Laboratory, Institute of Infectious Diseases, University of Pavia, IRCCS Policlinico S. Matteo, 27100 Pavia and 2 Central Research Laboratory, Sorin Biomedica SpA, 13040 Saluggia, Italy Using murine monoclonal antibodies (MAbs) to rubella virus haemagglutinin, five epitopes were identified in competitive ELISA binding assays: A, B, D and E by haemagglutination-inhibiting (HI) MAbs with no neutralizing (Nt) activity, and C by a MAb with neither activity. However, when HI and Nt activities were determined in the presence of anti-mouse immunoglobulins, epitopes A, B and D were defined by both HI and Nt MAbs, whereas epitopes C and E were identified by HI MAbs without Nt activity. A synergistic Nt activity, in the absence of anti-mouse immunoglobulins, was displayed by mixtures of antibodies of different epitope groups. Analysis of mixtures of MAb pairs each belonging to a different epitope class, showed that synergistic Nt activity was elicited primarily by the group A epitope, secondarily by groups B and D and only minimally by groups C and E. Keywords: rubella virus, haemagglutinin, monoclonal antibodies Received 22 December 1986; accepted 31 March 1987.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-68-7-2007