Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family

Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the...

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Bibliographic Details
Published in:Nature (London) 1987-07, Vol.328 (6127), p.221-227
Main Authors: Schofield, Peter R, Darlison, Mark G, Fujita, Norihisa, Burt, David R, Stephenson, F. Anne, Rodriguez, Henry, Rhee, Lucy M, Ramachandran, J, Reale, Vincenzina, Glencorse, Thora A, Seeburg, Peter H, Barnard, Eric A
Format: Article
Language:English
Subjects:
Animals
Cattle
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA - genetics
gamma-Aminobutyric Acid - physiology
Gene Expression Regulation
Genetics
Membrane Proteins - genetics
Multigene Family
Oocytes - physiology
Protein Conformation
Receptors, GABA-A - genetics
Receptors, GABA-A - physiology
Receptors, Nicotinic - genetics
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Summary:Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated alpha-subunit and beta-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.
ISSN:0028-0836
1476-4687
DOI:10.1038/328221a0