α-h-ANP is the only form of circulating ANP in humans

• Published in: FEBS letters 1987-06, Vol.218 (1), p.159-162
• Main Authors: Theiss, G., John, A., Morich, F., Neuser, D., Schröder, W., Stasch, J.-P., Wohlfeil, S.
• Format: Article
• Language: English
• Subjects: Adult; Amino acid analysis; Amino Acid Sequence; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Antibodies, Monoclonal - immunology; Atrial Natriuretic Factor - blood; Atrial Natriuretic Factor - immunology; Atrial Natriuretic Factor - isolation & purification; Atrial Natriuretic Factor - metabolism; Atrial natriuretic peptide; Biological and medical sciences; Chromatography, Affinity; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; Human plasma; Humans; Immunoaffinity chromatography; Peptide Fragments - analysis; Protein Precursors - metabolism; Proteins
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(87)81038-4

α-human atrial natriuretic peptide (α-h-ANP) was purified to homogeneity from human plasma of healthy adults in a three-step procedure including immunoaffinity chromatography on immobilized monoclonal anti-α-h-ANP antibody (moab). A single peak of immunoreactivity was obtained after final reversed-phase HPLC and the amino acid sequence of the isolated material was identical to that of synthetic α-h-ANP. No further atrial peptides could be detected although the moab reacts with all biologically active peptides. It is therefore concluded that α-h-ANP is the only form of ANP circulating in human plasma.