Detection of Minimal Residual Cells Carrying the t(14;18) by DNA Sequence Amplification

By means of the polymerase chain reaction (PCR) technique, DNA sequences were amplified that flank the crossover sites of a characteristic chromosomal translocation for follicular lymphomas, t(14;18)(q32;q21). This technique permitted the detection of cells carrying the t(14;18) hybrid DNA sequences...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-07, Vol.237 (4811), p.175-178
Main Authors: Lee, Ming-Sheng, Chang, Kun-Sang, Cabanillas, Fernando, Freireich, Emil J., Trujillo, Jose M., Stass, Sanford A.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Biotechnology
Bone marrow
Cancer
Cancer treatment
Care and treatment
Chromosomes
Chromosomes, Human, Pair 14 - ultrastructure
Chromosomes, Human, Pair 18 - ultrastructure
Disease remission
DNA
DNA polymerases
DNA, Neoplasm - analysis
Follicular lymphoma
Gene Amplification
Genetic Markers
Hematologic and hematopoietic diseases
Humans
Hybridity
Immunoglobulin J-Chains - genetics
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymphadenitis - genetics
Lymphoma, Follicular - genetics
Lymphomas
Medical research
Medical sciences
Neoplasm Recurrence, Local - diagnosis
Nucleotide sequences
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - genetics
Polymerase chain reaction
Relapse
Residual neoplasm
Testing
Translocation, Genetic
Tumors
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Description
Summary:By means of the polymerase chain reaction (PCR) technique, DNA sequences were amplified that flank the crossover sites of a characteristic chromosomal translocation for follicular lymphomas, t(14;18)(q32;q21). This technique permitted the detection of cells carrying the t(14;18) hybrid DNA sequences at a dilution of 1:100,000. The remission marrow and blood samples of a patient with follicular lymphoma and the t(14;18) failed to show any abnormality by morphological examination and conventional Southern blot analysis. However, the t(14;18) hybrid DNA sequences were detected by the PCR technique. Thus, this technique is a highly sensitive tool to detect minimal residual cells carrying the t(14;18) and has the potential to identify a subpopulation of patients with subclinical disease.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3110950