Non-selective cationic channel in primary cultured cells of brown adipose tissue

Single channel current events were recorded from membranes of cultured brown adipose tissue cells with the patch-clamp technique. In excised inside-out patches the predominant type of events showed a slope conductance of about 30 pS (25 degrees C). The current was carried by both, Na+ ions and K+ io...

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Bibliographic Details
Published in:Pflügers Archiv 1987-05, Vol.408 (5), p.534-536
Main Authors: SIEMEN, D, REUHL, T
Format: Article
Language:English
Subjects:
Adipose Tissue, Brown - cytology
Adipose Tissue, Brown - metabolism
Animals
Biological and medical sciences
Cells, Cultured
Cricetinae
Electrophysiology
Fundamental and applied biological sciences. Psychology
Ion Channels - metabolism
Potassium - metabolism
Sodium - metabolism
Temperature
Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue
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Summary:Single channel current events were recorded from membranes of cultured brown adipose tissue cells with the patch-clamp technique. In excised inside-out patches the predominant type of events showed a slope conductance of about 30 pS (25 degrees C). The current was carried by both, Na+ ions and K+ ions. Discrimination between them was poor. The frequency of events increased with increasing temperature. Their amplitude was temperature dependent as well (Q10 approximately equal to 1.4). A single exponential was not sufficient for fitting the histograms of "on-time" or "off-time". We conclude that these events belong to a type of non-selective cation channel described previously for other tissues.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00585082