Ultrafast protein determinations using microwave enhancement

We present here microwave-based modifications of standard protein assays that dramatically reduce the time required to determine protein concentrations. Typical protein determinations involve incubation times ranging from 15-60 min. Microwave irradiation of specimens reduces this time requirement to...

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Bibliographic Details
Published in:Molecular biotechnology 1995-08, Vol.4 (1), p.17-24
Main Authors: AKINS, R. E, TUAN, R. S
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biotechnology
Colorimetry - methods
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
Methods. Procedures. Technologies
Microwaves
Others
Proteins - analysis
Proteins - chemistry
Quinolines - chemistry
Various methods and equipments
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Summary:We present here microwave-based modifications of standard protein assays that dramatically reduce the time required to determine protein concentrations. Typical protein determinations involve incubation times ranging from 15-60 min. Microwave irradiation of specimens reduces this time requirement to 10-20 s without compromising accuracy or reliability. The remarkable speed with which protein determinations may be carried out using microwave enhancement greatly simplifies general laboratory procedures that depend on the estimation of protein concentrations.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02907468