Novel Assay System Favorable for the Study of Cell-to-Cell Transmission of HIV-1 and Its Application to the Evaluation of Anti-HIV Drugs

The cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) was studied using MOLT-4 cells chronically infected with a variant strain of HIV-1SF-2 (MOLT-4/HIV-1SF-2H) and CD4+ human lymphoid MT-4 cells. MOLT-4/HIV-1SF-2H cells produced less than 1 TCID50 infectious particles per 105...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1995/06/15, Vol.18(6), pp.920-922
Main Authors: INOUYE, Yoshio, KANAMORI, Tatsuyuki, FUJIMOTO, Yasuhiro, SUGIYAMA, Masanori, YOSHIDA, Tetsuya
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - drug therapy
Acquired Immunodeficiency Syndrome - virology
AIDS/HIV
Antiviral Agents - pharmacology
Biological and medical sciences
Cell Line
Cell-Free System
cell-to-cell transmission
Cytopathogenic Effect, Viral
de novo DNA synthesis
Drug Evaluation, Preclinical
Fundamental and applied biological sciences. Psychology
HIV-1 - drug effects
human immunodeficiency virus
human immunodeficiency virus 1
Humans
Microbiology
Polymers - pharmacology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Silicon Compounds - pharmacology
syncytium formation
Tungsten Compounds - pharmacology
Ultraviolet Rays
Virology
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Summary:The cell-to-cell transmission of human immunodeficiency virus type 1 (HIV-1) was studied using MOLT-4 cells chronically infected with a variant strain of HIV-1SF-2 (MOLT-4/HIV-1SF-2H) and CD4+ human lymphoid MT-4 cells. MOLT-4/HIV-1SF-2H cells produced less than 1 TCID50 infectious particles per 105 cells per day as determined by the cytopathogenicity in MT-4 cells. However, the expression of envelope glycoproteins gp120 and gp41 on the MOLT-4/HIV-1SF-2H cell membrane was satisfactory for syncytium formation with the uninfected MOLT-4 cells. When MOLT-4/HIV-1SF-2H and MT-4 cells were co-cultured, severe cytopathogenicity was observed in MT-4 cells without being accompanied by the formation of multi-nucleated cells. Thus, the system consisting of MOLT-4/HIV-1SF-2H and MT-4 cells is convenient for exclusive study of the mechanism of cell-to-cell transmission of HIV-1. Using various compounds, it was confirmed that cell-to-cell transmission required both gp120/gp41-CD4 binding and de novo DNA synthesis.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.18.920