Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli

The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated spectrofluorometrically. The presence of DFP during the loading per...

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Bibliographic Details
Published in:FEBS letters 1987-08, Vol.220 (1), p.89-92
Main Authors: Maruyama, Ikuo, Oyamada, Hideto, Hasegawa, Tomohiko, Ohtsuka, Kazuko, Momose, Kazutaka
Format: Article
Language:English
Subjects:
Aminoquinolines - metabolism
Animals
Benzofurans - metabolism
Biological and medical sciences
Calcium - metabolism
Diisopropyl fluorophosphate
Fluorescent Dyes - metabolism
Fura 2
Fura-2 - analogs & derivatives
Guinea Pigs
Hydrolysis
In Vitro Techniques
Isoflurophate - pharmacology
Medical sciences
Muscle
Muscle, Smooth - drug effects
Pharmacology. Drug treatments
Quin 2
Single smooth muscle cell, Guinea pig taenia coli
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Summary:The effect of diisopropyl fluorophosphate (DFP), a potent cholinesterase (ChE) inhibitor, on loading quin 2 acetoxymethyl ester (quin 2/AM) and fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated spectrofluorometrically. The presence of DFP during the loading permitted the incorporation of quin 2 into the cells, so that it became possible to measure intracellular Ca 2+ concentrations using the ester of this dye. Also, DFP significantly enhanced the incorporation of fura 2 into the cells. These results indicate that loading of quin 2/AM and fura 2/AM into the smooth muscle cells may depend on the suppression of ChE or various serine protease activities outside cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80881-5