Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE)

The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, ha...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biotechnology 1995-08, Vol.4 (1), p.25-43
Main Authors: Noolandi, J. (Xerox Research Centre of Canada, Mississauga, Ontario, Canada.), Turmel, C
Format: Article
Language:English
Subjects:
ADN
Algorithms
Biological and medical sciences
Biotechnology
Blotting, Southern
Buffers
Candida - genetics
Candida albicans - genetics
CHROMOSOME
CHROMOSOMES
Chromosomes - chemistry
CROMOSOMAS
Diverse techniques
DNA
DNA - chemistry
DNA - metabolism
DNA, Fungal - chemistry
ECHANTILLONNAGE
Electricity
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
Electrophoresis, Gel, Pulsed-Field - instrumentation
Electrophoresis, Gel, Pulsed-Field - methods
ENDOMYCETALES
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
large dna molecules
Methods. Procedures. Technologies
Miscellaneous
Molecular and cellular biology
MUESTREO
PICHIA
PICHIA STIPITIS
PROCESAMIENTO
PROCESSING
PULSED FIELD ELECTROPHORESIS
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
SAMPLING
schizosaccharomyces pombe
Synthetic digonucleotides and genes. Sequencing
TRAITEMENT
zero integrated field electrophoresis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, hardware requirements, and procedures are given. A variety of gel pictures for known yeast DNA markers are shown.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02907469