Effects of Roxithromycin on Proliferation of Peripheral Blood Mononuclear Cells and Production of Lipopolysaccharide-Induced Cytokines

• Published in: Biological & pharmaceutical bulletin 1995/06/15, Vol.18(6), pp.876-881
• Main Authors: YOSHIMURA, Tomoaki, KURITA, Chikako, YAMAZAKI, Futoshi, SHINDO, Joe, MORISHIMA, Itsuro, MACHIDA, Kazuya, SUMITA, Toshiaki, HORIBA, Michiaki, NAGAI, Hiroichi
• Format: Article
• Language: English
• Subjects: adherent cell; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Cell Adhesion - drug effects; Cell Division - drug effects; Cytokines - biosynthesis; Humans; In Vitro Techniques; interleukin 1β; Interleukin-1 - biosynthesis; Lipopolysaccharides - pharmacology; macrolide; Medical sciences; monocyte/macrophage; Monocytes - drug effects; Monocytes - metabolism; Pharmacology. Drug treatments; Phytohemagglutinins - pharmacology; roxithromycin; Roxithromycin - pharmacology; tumor necrosis factor α; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.18.876

Roxithromycin (RXM), a new macrolide antibiotic, has a 14-member macrocycline ring structure which is similar to that of erythromycin. We investigated the effects of RXM on the proliferation of peripheral blood mononuclear cells (PBMCs) and the production of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) by PBMCs stimulated with lipopolysaccharide (LPS). At concentrations greater than 25.0 μg/ml, RXM suppressed the proliferation of PBMCs stimulated with phytohemagglutinin, probably due to cytotoxicity. When the PBMCs were incubated with RXM for 7d, the number of adherent cells (monocyte/macrophages) increased. Incubation with RXM at a concentration of 25.0 μg/ml induced the greatest increase (p