Babesia bovis: vaccination studies with three groups of high molecular weight antigens from lysate of infected erythrocytes

A void volume fraction and fractions of mean sizes 800 kdalton and 300 kdalton were isolated by gel filtration from lysate of bovine erythrocytes infected with Babesia bovis. All fractions had good serological activity, as assayed by ELISA and IFA. Groups of four splenectomized calves were vaccinate...

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Bibliographic Details
Published in:Parasitology research (1987) 1987, Vol.73 (4), p.319-323
Main Authors: WALTISBUHL, D. J, GOODGER, B. V, WRIGHT, I. G, MIRRE, G. B, COMMINS, M. A
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
Antigens, Protozoan - immunology
Babesia - immunology
Babesiosis - prevention & control
Biological and medical sciences
Cattle
Cattle Diseases - prevention & control
Chromatography, Gel
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Erythrocytes - immunology
Erythrocytes - parasitology
Fluorescent Antibody Technique
Infectious diseases
Medical sciences
Parasitic diseases
Protozoal diseases
Vaccination - veterinary
Vaccines
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Summary:A void volume fraction and fractions of mean sizes 800 kdalton and 300 kdalton were isolated by gel filtration from lysate of bovine erythrocytes infected with Babesia bovis. All fractions had good serological activity, as assayed by ELISA and IFA. Groups of four splenectomized calves were vaccinated with each fraction and then challenged, together with groups of four control calves, with a homologous strain of B. bovis. The group vaccinated with the 300 kdalton fraction showed some protection, as indicated by delayed and significantly lower parasitaemias and by a 75% survival in the group. In contrast, all animals in the relevant control group died from infection. No evident protection was obtained with the other two fractions.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00531085