Detection of pathogenic fungi in human blood by the polymerase chain reaction

The ability of the polymerase chain reaction (PCR) to detect pathogenic fungi in human blood was investigated. A DNA fragment of about 300 bp from the 18S rDNA, highly conserved in all fungi, was amplified with target DNA from 18 different species of fungi commonly isolated from clinical samples. Th...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1995-07, Vol.14 (7), p.618-621
Main Authors: POLANCO, A. M, RODRIGUEZ-TUDELA, J. L, MARTINEZ-SUAREZ, J. V
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Candida albicans
Fundamental and applied biological sciences. Psychology
Fungemia - diagnosis
Fungi - isolation & purification
Humans
Microbiology
Molecular Sequence Data
Mycological methods and techniques used in mycology
Mycology
Polymerase Chain Reaction
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Summary:The ability of the polymerase chain reaction (PCR) to detect pathogenic fungi in human blood was investigated. A DNA fragment of about 300 bp from the 18S rDNA, highly conserved in all fungi, was amplified with target DNA from 18 different species of fungi commonly isolated from clinical samples. The presence of PCR products was confirmed by hybridization with a fluorescein-labelled internal probe (21-mer). The PCR assay described is sensitive enough to detect 125 fg of purified Candida albicans DNA and 10 to 100 yeast cells per millilitre of blood.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF01690738