Compositions of very low density lipoprotein subfractions from patients with polydisperse low density lipoproteins

In some hyperlipidemic patients, low density lipoprotein (LDL) shows several peaks (polydisperse) on polyacrylamide gel disc electrophoreses, though LDL usually shows a single peak (monodisperse). In order to clarify the relationship between the LDL polydispersion and VLDL heterogeneity, LDL and VLD...

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Bibliographic Details
Published in:Clinica chimica acta 1995-06, Vol.238 (1), p.43-57
Main Authors: Saheki, Shuichi, Hitsumoto, Yasuo, Bando, Shiro, Murase, Mitsuharu, Takeuchi, Nozomu, Uchida, Kiyohisa
Format: Article
Language:English
Subjects:
Adult
Apolipoproteins - blood
Apoprotein
Biological and medical sciences
Centrifugation, Density Gradient
Cholesterol - blood
Disorders of blood lipids. Hyperlipoproteinemia
Electrophoresis, Polyacrylamide Gel
Female
Gradient ultracentrifugation
Humans
LDL heterogeneity
Lipoprotein composition
Lipoproteins, LDL - chemistry
Lipoproteins, VLDL - chemistry
Male
Medical sciences
Metabolic diseases
Middle Aged
Triglycerides - blood
VLDL subfraction
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Summary:In some hyperlipidemic patients, low density lipoprotein (LDL) shows several peaks (polydisperse) on polyacrylamide gel disc electrophoreses, though LDL usually shows a single peak (monodisperse). In order to clarify the relationship between the LDL polydispersion and VLDL heterogeneity, LDL and VLDL were prepared from hyperlipidemic patients sera with mono- and polydisperse LDL by sequential ultracentrifugation and fractionated by gradient ultracentrifugation and their compositions were analyzed. Polydisperse LDL was rich in triacylglycerol (TG) and poor in esterified cholesterol (CE) as compared with monodisperse LDL and consisted of the lowest and the medium density subfractions when the LDL was separated into six subfractions. The monodisperse LDL was composed of a single major subfraction of a medium density. VLDL from the patients with polydisperse LDL was relatively rich in the dense and poor in the buoyant subfractions as compared with that from the patients with monodisperse LDL. The subfractions in the former contained more CE and less TG than the corresponding subfractions in the latter. There were no significant differences in the apolipoprotein compositions between those VLDLs. The results suggest that polydisperse LDL might be originated from VLDL that differs in particle sizes, densities and compositions from ordinary VLDL.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(95)06075-O