A highly sensitive and rapid procedure for direct PCR detection of Leishmania infantum within human peripheral blood mononuclear cells

We have developed a highly sensitive, simple and rapid procedure to detect Leishmania infantum within human macrophages. It only requires ficoll preparation of peripheral blood mononuclear cells from the patient, and their direct use for Leishmania kDNA amplification by polymerase chain reaction. Un...

Full description

Saved in:
Bibliographic Details
Published in:Acta tropica 1995-06, Vol.59 (3), p.187-196
Main Authors: Ravel, Sophie, Cuny, Gérard, Reynes, Jacques, Veas, Francisco
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Diagnosis
Human protozoal diseases
Humans
Infectious diseases
Leishmania
Leishmania infantum - isolation & purification
Leshmaniasis
Leukocytes, Mononuclear - parasitology
Macrophages - parasitology
Medical sciences
Molecular Sequence Data
Parasitic diseases
PBMCs
PCR
Polymerase Chain Reaction
Protozoal diseases
Sensitivity
Sensitivity and Specificity
Tropical medicine
Visceral Leishmaniasis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a highly sensitive, simple and rapid procedure to detect Leishmania infantum within human macrophages. It only requires ficoll preparation of peripheral blood mononuclear cells from the patient, and their direct use for Leishmania kDNA amplification by polymerase chain reaction. Under these conditions, about one parasite can be detected in a one million human cell environment. Results, including those of a hybridization step to confirm the diagnosis specificity. are obtained within 24h, a very short period as compared to current diagnostic methods. This procedure is of particular interest for early detection and early drug treatment of leishmaniasis, especially in the case of HIV coinfection. Furthermore the method could be useful for monitoring the efficiency of new leishmaniasis treatments in infected patients.
ISSN:0001-706X
1873-6254
DOI:10.1016/0001-706X(95)00079-T