Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool

Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals bu...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1995-10, Vol.374 (1), p.29-33
Main Authors: Halbwachs-Mecarelli, L., Bessou, G., Lesavre, P., Lopez, S., Witko-Sarsat, V.
Format: Article
Language:English
Subjects:
ANCA
ANCA, antineutrophil cytoplasmic autoantibodies
Animals
Arthritis, Rheumatoid - immunology
Autoantibodies - immunology
Blood Donors
Cell Membrane - enzymology
cyt. B, cytochalasin B
fMLP, N-formyl-methionyl-leucyl-phenylalanine
Granulomatosis with Polyangiitis - immunology
HBSS, Hanks' balanced salt solution
Humans
Mice
Myeloblastin
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils - enzymology
Neutrophils - immunology
Phenotype
PMN serine protease
PMN, polymorphonuclear neutrophils
Polymorphonuclear neutrophil
PR3, proteinase 3
Proteinase 3
Serine Endopeptidases - classification
Serine Endopeptidases - metabolism
Vasculitis
WG, Wegener's granulomatosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. The existence of a subset of PMN which spontaneously expresses PR3 and varies among individuals, may be relevant to the pathogenesis of anti-PR3 ANCA autoantibody-related vasculitis.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01073-N