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Lead inhibits Ca(2+)-stimulated nitric oxide synthase activity from rat cerebellum

Pb2+ is reported to cause cognitive dysfunctions in children and to inhibit long-term potentiation (LTP), a model form of synaptic plasticity that involves nitric oxide (NO). Since Pb2+ interacts with Ca(2+)-calmodulin, and brain nitric oxide synthase (NOS) is Ca(2+)-calmodulin regulated, we examine...

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Published in:	Neuroscience letters 1995-08, Vol.196 (1-2), p.65-68
Main Authors:	Quinn, M R, Harris, C L
Format:	Article
Language:	English
Subjects:	Animals Calcium - pharmacology Cations - pharmacology Cerebellum - drug effects Dose-Response Relationship, Drug Egtazic Acid - pharmacology Lead - pharmacology Male NADP - pharmacology Nitric Oxide Synthase - drug effects Rats Rats, Sprague-Dawley
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container_title	Neuroscience letters
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creator	Quinn, M R Harris, C L
description	Pb2+ is reported to cause cognitive dysfunctions in children and to inhibit long-term potentiation (LTP), a model form of synaptic plasticity that involves nitric oxide (NO). Since Pb2+ interacts with Ca(2+)-calmodulin, and brain nitric oxide synthase (NOS) is Ca(2+)-calmodulin regulated, we examined the effects of Pb2+ on NOS activity prepared from rat cerebellum. NOS required NADPH and was inhibited by monomethylarginine. Full NOS activity required 0.6 microM free Ca2+ and was inhibited 50% by 17 nM and 100% by 80 nM free Pb2+. NOS inhibition by Pb2+ was reversible by increasing free Ca2+ concentrations. Evaluation of other divalent cations resulted in the following ranked order of potencies: Cu2+ > Pb2+ >> Zn2+; Fe2+, Ba2+, Mg2+, Mn2+, and Sr2+ were ineffective. These results suggest that Pb2+ inhibition of brain NOS activity may account for some of the effects of Pb2+ on the CNS.
doi_str_mv	10.1016/0304-3940(95)11845-N
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subjects	Animals Calcium - pharmacology Cations - pharmacology Cerebellum - drug effects Dose-Response Relationship, Drug Egtazic Acid - pharmacology Lead - pharmacology Male NADP - pharmacology Nitric Oxide Synthase - drug effects Rats Rats, Sprague-Dawley
title	Lead inhibits Ca(2+)-stimulated nitric oxide synthase activity from rat cerebellum
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