The Effects of Three Recombinant Growth Factors, IL-3, GM-CSF, and G-CSF, on the Blast Cells of Acute Myeloblastic Leukemia Maintained in Short-Term Suspension Culture

The blast stem cells of acute myeloblastic leukemia (AML) respond in cell culture to growth factors by both self-renewal and terminal divisions. Both of these functions have been shown to be stimulated by the recombinant growth factors granulocyte-macrophage colony-stimulat-ing factor (GM-CSF) and g...

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Bibliographic Details
Published in:Blood 1987-09, Vol.70 (3), p.657-663
Main Authors: Miyauchi, Jun, Kelleher, Colm A., Yang, Yu-Chung, Wong, Gordon G., Clark, Steven C., Minden, Mark D., Minkin, Salomon, McCulloch, Ernest A.
Format: Article
Language:English
Subjects:
Acute Disease
Biological and medical sciences
Blood Cells - drug effects
Blood Cells - pathology
Bone Marrow - drug effects
Bone Marrow Cells
Cell Count
Cells, Cultured
Cytological Techniques
Hematologic and hematopoietic diseases
Humans
Interleukin-3 - pharmacology
Leukemia, Myeloid, Acute - blood
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Methylcellulose
Recombinant Proteins - pharmacology
Recombination, Genetic
Tumor Stem Cell Assay
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Summary:The blast stem cells of acute myeloblastic leukemia (AML) respond in cell culture to growth factors by both self-renewal and terminal divisions. Both of these functions have been shown to be stimulated by the recombinant growth factors granulocyte-macrophage colony-stimulat-ing factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). In this paper, recombinant gibbon inter leu-kin-3 (IL-3), homologous to human IL-3, was tested on blast cells and compared with the effects of GM-CSF, G-CSF, and medium conditioned by the bladder cell line 5637 (5637-CM). We found that IL-3 was an effective stimulator of blast renewal and terminal divisions. However, great patient-to-patient variation was found. A graphic method of presenting complex comparisons between growth factors is also included.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V70.3.657.657