Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis

A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1995, Vol.39(8), pp.619-622
Main Authors: Matsuyama, Junko, Yamamoto, Koichiro, Miwatani, Toshio, Honda, Takeshi
Format: Article
Language:English
Subjects:
Antibodies, immunoglobulins
Antibodies, Monoclonal - immunology
Bacillus cereus
Bacillus thuringiensis
Bacillus thuringiensis - metabolism
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hemolysin
Hemolysin Proteins - immunology
Hybridomas
Molecular immunology
Monoclonal antibodies
Monoclonal antibody
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Summary:A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by inununoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1995.tb02251.x