Porcine Submaxillary Gland GDP-L-fucose:β-D-Galactoside α-2-L-Fucosyltransferase Is Likely a Counterpart of the Human Secretor Gene-encoded Blood Group Transferase (∗)

Partial amino acid sequence of GDP-L-fucose:β-D-galactoside α-2-L-fucosyltransferase purified from porcine submaxillary glands was determined. Amino acid sequence analysis yielded 100, 93.3, and 84.2%, and 75, 46.6, and 84.2% sequence identity between 12-, 15-, and 19-amino acid tryptic peptides gen...

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Bibliographic Details
Published in:The Journal of biological chemistry 1995-11, Vol.270 (44), p.26577-26580
Main Authors: Thurin, Jan, Blaszczyk-Thurin, Magdalena
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Chromatography, Affinity
Chromatography, Gel
Chromatography, High Pressure Liquid
Fucosyltransferases - chemistry
Fucosyltransferases - genetics
Fucosyltransferases - isolation & purification
Galactoside 2-alpha-L-fucosyltransferase
Humans
Molecular Sequence Data
Peptide Fragments - chemistry
Peptide Fragments - isolation & purification
Submandibular Gland - enzymology
Swine
Trypsin
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Summary:Partial amino acid sequence of GDP-L-fucose:β-D-galactoside α-2-L-fucosyltransferase purified from porcine submaxillary glands was determined. Amino acid sequence analysis yielded 100, 93.3, and 84.2%, and 75, 46.6, and 84.2% sequence identity between 12-, 15-, and 19-amino acid tryptic peptides generated from porcine enzyme and amino acid residues 61-72, 111-125, and 308-326 and 89-100, 139-153, and 338-356 of the human Secretor and H type α-2-fucosyltransferases, respectively. Higher amino acid sequence homology of the porcine enzyme with the predicted sequence for the human Secretor locus as compared with H gene-encoded blood group β-D-galactoside α-2-L-fucosyltransferase suggests that porcine α-2-fucosyltransferase highly corresponds to the human Secretor gene-encoded enzyme.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.270.44.26577