Isolation and identification of Lom-SG-SASP, a salivation stimulating peptide from the salivary glands of Locusta migratoria

From a methanolic extract of about 2500 salivary glands of Locusta migratoria a peptide was isolated which stimulates cAMP production in the salivary glands and salivation. Maldi-TOFMS revealed a mass of 1779 Da. The primary structure of the peptide is NH 2-EVGDLFKEWLQGNMN-COOH. The peptide is named...

Full description

Saved in:
Bibliographic Details
Published in:Regulatory peptides 1995-06, Vol.57 (3), p.221-226
Main Authors: Veelaert, Dirk, Schoofs, Liliane, Proost, Paul, Van Damme, Jo, Devreese, Bart, Van Beeumen, Jozef, De Loof, Arnold
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cyclic AMP - biosynthesis
Endocrinology
Grasshoppers - chemistry
Insect
Molecular Sequence Data
Molecular Weight
Neuropeptide
Peptide
Salivary Proteins and Peptides - isolation & purification
Salivary Proteins and Peptides - physiology
Salivation
Salivation - physiology
Second messenger
Water balance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:From a methanolic extract of about 2500 salivary glands of Locusta migratoria a peptide was isolated which stimulates cAMP production in the salivary glands and salivation. Maldi-TOFMS revealed a mass of 1779 Da. The primary structure of the peptide is NH 2-EVGDLFKEWLQGNMN-COOH. The peptide is named Locusta migratoria-Salivary Gland-Salivation Stimulating Peptide (Lom-SG-SASP) because of its simulating effect on salivation. Lom-SG-SASP displays no relevant sequence similarities with any other known peptide from vertebrate or invertebrate sources. The effect of synthetic Lom-SG-SASP on cAMP production in the salivary glands and on salivation is discussed.
ISSN:0167-0115
1873-1686
DOI:10.1016/0167-0115(95)00035-A