Generation of hydrogen peroxide by brown adipose tissue mitochondria

This is the first report on the generation of H2O2 by brown adipose tissue mitochondria. Flavin dehydrogenase-linked substrates like succinate, glycerol-1-phosphate, and fatty acyl CoA were good substrates for the reaction, while NAD+-linked substrates were less effective. In cold-acclimated animals...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bioenergetics and biomembranes 1987-08, Vol.19 (4), p.397-407
Main Authors: Sekhar, B S, Kurup, C K, Ramasarma, T
Format: Article
Language:English
Subjects:
adipose tissue (brown)
Adipose Tissue, Brown - metabolism
Animals
Cations
Cold Temperature
hydrogen peroxide
Hydrogen Peroxide - metabolism
Kinetics
Male
mitochondria
Mitochondria - metabolism
Rats
Rats, Inbred Strains
Substrate Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This is the first report on the generation of H2O2 by brown adipose tissue mitochondria. Flavin dehydrogenase-linked substrates like succinate, glycerol-1-phosphate, and fatty acyl CoA were good substrates for the reaction, while NAD+-linked substrates were less effective. In cold-acclimated animals the activity showed a substantial increase (2.5-fold). The Km and Vmax of the reaction were considerably lower than those of the respective dehydrogenase. Metal ions, particularly Cu2+ and Fe2+ were potent inhibitors of the reaction. Nucleoside diphosphates, which were inhibitors by themselves, potentiated the inhibitory action of Fe2+ ions. In most of the properties, the H2O2 generator of brown adipose tissue mitochondria resembled that of liver mitochondria.
ISSN:0145-479X
1573-6881
DOI:10.1007/BF00768542