Sequential appearance of epidermal growth factor in plasma membrane-associated and intracellular vesicles during endocytosis

Receptor-mediated internalization of epidermal growth factor (EGF) occurs by a process involving initially clathrin-coated pits on the cell surface and the subsequent formation of ligand-containing endosomes. Using a modified acid wash technique, cell surface-bound EGF was removed. Utilizing sucrose...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-08, Vol.262 (24), p.11407-11409
Main Authors: Hertel, C, Perkins, J P
Format: Article
Language:English
Subjects:
astrocytoma
Astrocytoma - ultrastructure
Cell Line
Cell Membrane - analysis
Endocytosis
epidermal growth factor
Epidermal Growth Factor - analysis
ErbB Receptors - metabolism
Humans
Hydrogen-Ion Concentration
man
Organoids - analysis
Temperature
Time Factors
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Summary:Receptor-mediated internalization of epidermal growth factor (EGF) occurs by a process involving initially clathrin-coated pits on the cell surface and the subsequent formation of ligand-containing endosomes. Using a modified acid wash technique, cell surface-bound EGF was removed. Utilizing sucrose density centrifugation, the residual cell-associated EGF was separated into plasma membrane-associated and intracellular vesicle-associated forms. Using these procedures we have identified a transient form of cell-associated EGF that is still attached to the plasma membrane but not accessible to the extracellular fluid. This form of EGF appears to be the precursor for endosomic EGF. We suggest that this intermediate form represents the receptor-ligand complex shown by electronmicroscopy to be located in narrow-necked plasma membrane invaginations (Willingham, M. C., and Pastan, I. (1980) Cell 21, 67-77).
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)60820-8