Clonal analysis using recombinant DNA probes from X-chromosome

It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particula...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1987-09, Vol.47 (18), p.4806-4813
Main Authors: VOGELSTEIN, B, FEARON, E. R, HAMILTON, S. R, PREISINGER, A. C, WILLARD, H. F, MICHELSON, A. M, RIGGS, A. D, ORKIN, S. H
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
Biological and medical sciences
Chromatin. Chromosome
DNA, Neoplasm - analysis
DNA, Recombinant
Female
Fundamental and applied biological sciences. Psychology
Humans
Hypoxanthine Phosphoribosyltransferase - genetics
Methylation
Molecular and cellular biology
Molecular genetics
Neoplasms - genetics
Phosphoglycerate Kinase - genetics
Polymorphism, Restriction Fragment Length
X Chromosome
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Summary:It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.
ISSN:0008-5472
1538-7445