Modification of a tunable UV-visible capillary electrophoresis detector for simultaneous absorbance and fluorescence detection: profiling of body fluids for drugs and endogenous compounds

Using fused-silica optical fibres for fluorescence light collection and bandpass filters for selection of emission wavelengths, a capillary electrophoresis detection cell of a conventional, tunable UV-Vis absorbance detector was adapted for simultaneous fluorescence (at selected emission wavelength)...

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Bibliographic Details
Published in:Journal of Chromatography A 1995-08, Vol.709 (1), p.147-156
Main Authors: Caslavska, Jitka, Gassmann, Ernst, Thormann, Wolfgang
Format: Article
Language:English
Subjects:
Catecholamines - analysis
Catecholamines - blood
Catecholamines - urine
Chromatography, High Pressure Liquid
Electrophoresis - methods
Fluorescence Polarization Immunoassay
Humans
Indoles - analysis
Indoles - blood
Indoles - urine
Naproxen - analysis
Naproxen - blood
Naproxen - urine
Quinidine - blood
Quinidine - urine
Salicylates - blood
Salicylates - urine
Spectrophotometry, Ultraviolet - methods
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Summary:Using fused-silica optical fibres for fluorescence light collection and bandpass filters for selection of emission wavelengths, a capillary electrophoresis detection cell of a conventional, tunable UV-Vis absorbance detector was adapted for simultaneous fluorescence (at selected emission wavelength) and absorbance (at selected excitation wavelength) detection. Detector performance is demonstrated with the monitoring of underivatized fluorescent compounds in body fluids by micellar electrokinetic capillary chromatography with direct sample injection. Compared with UV absorption detection, fluorescence detection is shown to provide increased selectivity and for selected compounds also up to tenfold higher sensitivity. Examples studied include screening for urinary indole derivatives (tryptophan, 5-hydroxytryptophan, tyrosine, 3-indoxyl sulfate and 5-hydroxyindole-3-acetic acid) and catecholamine metabolites (homovanillic acid and vanillylmandelic acid) and the monitoring of naproxen in serum, quinidine in serum and urine and of salicylate and its metabolites in serum and urine.
ISSN:0021-9673
DOI:10.1016/0021-9673(95)00115-4