Properties of voltage-gated currents of microglia developed using macrophage colony-stimulating factor

Microglia were isolated from a murine neonatal brain cell culture in which their development had been stimulated by supplementation with the macrophage/microglial growth factor macrophage colony-stimulating factor (M-CSF). Using the whole-cell configuration of the patch-clamp technique, voltage-gate...

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Bibliographic Details
Published in:Pflügers Archiv 1995-08, Vol.430 (4), p.526-533
Main Authors: Eder, C, Fischer, H G, Hadding, U, Heinemann, U
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Barium - metabolism
Cations - metabolism
Cells, Cultured
Electrophysiology
Hydrogen-Ion Concentration
Ion Channel Gating - drug effects
Ion Channel Gating - physiology
Ion Channels - drug effects
Ion Channels - physiology
Macrophage Colony-Stimulating Factor - physiology
Membrane Potentials - drug effects
Mice
Mice, Inbred C3H
Microglia - drug effects
Microglia - metabolism
Microglia - physiology
Patch-Clamp Techniques
Potassium - physiology
Tetraethylammonium Compounds - pharmacology
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Summary:Microglia were isolated from a murine neonatal brain cell culture in which their development had been stimulated by supplementation with the macrophage/microglial growth factor macrophage colony-stimulating factor (M-CSF). Using the whole-cell configuration of the patch-clamp technique, voltage-gated membrane currents were recorded from these microglial cells. Hyperpolarization induced inward rectifying K+ currents, as described for microglia from untreated cultures. These currents activated negative to the K+ equilibrium potential and, with a strong hyperpolarization, displayed time-dependent inactivation. The inactivation was abolished when extracellular NaCl was replaced by N-methyl-D-glucamine (NMG), thereby indicating a partial block of this K+ conductance by Na+. Inward rectifying currents were also blocked by extracellularly applied Cs+ or Ba2+. They were slightly diminished following treatment with extracellular tetraethylammonium chloride (TEA) but were not affected by 4-aminopyridine (4-AP). Upon long lasting depolarizing voltage pulses to potentials positive to 0 mV, the cells exhibited a slowly activating H+ current which could be reduced by application of inorganic polyvalent cations (Ba2+, Cd2+, Co2+, La3+, Ni2+, Zn2+) as well as by 4-AP or TEA. Based on their kinetics and pharmacological characteristics, both currents detected on M-CSF-grown microglia are suggested to correspond to the inward rectifier and the H+ current of macrophages.
ISSN:0031-6768
1432-2013
DOI:10.1007/bf00373889