Dolichyl Phosphate, a Potent Inducer of Apoptosis in Rat Glioma C6 Cells

Exposure of rat glioma C6 cells to dolichyl phosphate resulted in cell shrinkage followed by nuclear fragmentation and internucleosomal cleavage of genomic DNA, yielding ladder patterns of oligonucleosomal fragments, all characteristics of apoptosis, This phenomenon occurred in a dose and time depen...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-11, Vol.216 (3), p.848-853
Main Authors: Yasugi, E., Yokoyama, Y., Seyama, Y., Kano, K., Hayashi, Y., Oshima, M.
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
DNA - metabolism
Dolichol - pharmacology
Dolichol Phosphates - pharmacology
Electrophoresis, Agar Gel
Glioma - metabolism
Glioma - pathology
Glycoproteins - antagonists & inhibitors
Glycosylation - drug effects
HeLa Cells
Humans
Kinetics
Pentanols - pharmacology
Rats
Swainsonine - pharmacology
Tumor Cells, Cultured
Tunicamycin - pharmacology
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Summary:Exposure of rat glioma C6 cells to dolichyl phosphate resulted in cell shrinkage followed by nuclear fragmentation and internucleosomal cleavage of genomic DNA, yielding ladder patterns of oligonucleosomal fragments, all characteristics of apoptosis, This phenomenon occurred in a dose and time dependent manner. Dolichol and prenol failed to induce apoptosis. The inhibitors of N-glycosylation, tunicamycin and swainsonine had no apparent effect on dolichyl phosphate-induced apoptosis. Apoptotic changes were also observed in HL-60 cells, SIRC cells and HeLa cells. Thus, dolichyl phosphate functions as a potential apoptosis inducer as well as an essential carrier lipid in the biosynthesis of N-linked glycoprotein.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2699